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spectral correction mtq2 (donor only)  (Addgene inc)


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    Structured Review

    Addgene inc spectral correction mtq2 (donor only)
    Expected <t>outcomes:</t> <t>Spectral</t> correction factors and FRET calibration (A) Determination of spectral correction factors: R A1 : Plot of fluorescence in the FRET channel (y-axis) vs. fluorescence in the YFP channel (x-axis). R A1 describes cross excitation of YFP by CFP excitation (FRET/YFP). R D1 : Plot of fluorescence in the FRET channel (y-axis) vs. fluorescence in the CFP channel (x-axis). R D1 describes the bleed-through of CFP emission into the FRET emission detection range (FRET/CFP). R <t>D2</t> : Plot of fluorescence in the YFP channel vs. the fluorescence in the CFP channel. R D2 describes cross excitation of CFP from the YFP excitation (YFP/CFP). These spectral correction factors are determined by linear regressions between the specified channels. (B) Correlating sensitized emission versus donor quenching displays an affine relationship between these quantities when using dimer constructs. The G ratio corresponds to the slope of a linear regression line, while the F ratio is calculated from the y-axis offset. (C) Left: cartoon of the dimer constructs. Right: Plot of FRET efficiencies determined from donor quenching ( E D ) vs. A free for the shown dimers. FRET calibration results in stable FRET efficiencies independent of the fluorophore concentration ( A free or D free ). The plot for sensitized emission ( E A ) should look similar. All calibration images were processed with the “FRET_PIX” macro instead of the “FRET_ROI” macro.
    Spectral Correction Mtq2 (Donor Only), supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/spectral+correction+mtq2+(donor+only)/pmturquoise2+golgi/pmc10400964-0-8-21
    Average 90 stars, based on 1 article reviews
    spectral correction mtq2 (donor only) - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Protocol for deriving proximity, affinity, and stoichiometry of protein interactions using image-based quantitative two-hybrid FRET"

    Article Title: Protocol for deriving proximity, affinity, and stoichiometry of protein interactions using image-based quantitative two-hybrid FRET

    Journal: STAR Protocols

    doi: 10.1016/j.xpro.2023.102459

    Expected outcomes: Spectral correction factors and FRET calibration (A) Determination of spectral correction factors: R A1 : Plot of fluorescence in the FRET channel (y-axis) vs. fluorescence in the YFP channel (x-axis). R A1 describes cross excitation of YFP by CFP excitation (FRET/YFP). R D1 : Plot of fluorescence in the FRET channel (y-axis) vs. fluorescence in the CFP channel (x-axis). R D1 describes the bleed-through of CFP emission into the FRET emission detection range (FRET/CFP). R D2 : Plot of fluorescence in the YFP channel vs. the fluorescence in the CFP channel. R D2 describes cross excitation of CFP from the YFP excitation (YFP/CFP). These spectral correction factors are determined by linear regressions between the specified channels. (B) Correlating sensitized emission versus donor quenching displays an affine relationship between these quantities when using dimer constructs. The G ratio corresponds to the slope of a linear regression line, while the F ratio is calculated from the y-axis offset. (C) Left: cartoon of the dimer constructs. Right: Plot of FRET efficiencies determined from donor quenching ( E D ) vs. A free for the shown dimers. FRET calibration results in stable FRET efficiencies independent of the fluorophore concentration ( A free or D free ). The plot for sensitized emission ( E A ) should look similar. All calibration images were processed with the “FRET_PIX” macro instead of the “FRET_ROI” macro.
    Figure Legend Snippet: Expected outcomes: Spectral correction factors and FRET calibration (A) Determination of spectral correction factors: R A1 : Plot of fluorescence in the FRET channel (y-axis) vs. fluorescence in the YFP channel (x-axis). R A1 describes cross excitation of YFP by CFP excitation (FRET/YFP). R D1 : Plot of fluorescence in the FRET channel (y-axis) vs. fluorescence in the CFP channel (x-axis). R D1 describes the bleed-through of CFP emission into the FRET emission detection range (FRET/CFP). R D2 : Plot of fluorescence in the YFP channel vs. the fluorescence in the CFP channel. R D2 describes cross excitation of CFP from the YFP excitation (YFP/CFP). These spectral correction factors are determined by linear regressions between the specified channels. (B) Correlating sensitized emission versus donor quenching displays an affine relationship between these quantities when using dimer constructs. The G ratio corresponds to the slope of a linear regression line, while the F ratio is calculated from the y-axis offset. (C) Left: cartoon of the dimer constructs. Right: Plot of FRET efficiencies determined from donor quenching ( E D ) vs. A free for the shown dimers. FRET calibration results in stable FRET efficiencies independent of the fluorophore concentration ( A free or D free ). The plot for sensitized emission ( E A ) should look similar. All calibration images were processed with the “FRET_PIX” macro instead of the “FRET_ROI” macro.

    Techniques Used: Fluorescence, Construct, Concentration Assay



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    spectral correction mtq2 (donor only)  (Addgene inc)
    90
    Addgene inc spectral correction mtq2 (donor only)
    Expected <t>outcomes:</t> <t>Spectral</t> correction factors and FRET calibration (A) Determination of spectral correction factors: R A1 : Plot of fluorescence in the FRET channel (y-axis) vs. fluorescence in the YFP channel (x-axis). R A1 describes cross excitation of YFP by CFP excitation (FRET/YFP). R D1 : Plot of fluorescence in the FRET channel (y-axis) vs. fluorescence in the CFP channel (x-axis). R D1 describes the bleed-through of CFP emission into the FRET emission detection range (FRET/CFP). R <t>D2</t> : Plot of fluorescence in the YFP channel vs. the fluorescence in the CFP channel. R D2 describes cross excitation of CFP from the YFP excitation (YFP/CFP). These spectral correction factors are determined by linear regressions between the specified channels. (B) Correlating sensitized emission versus donor quenching displays an affine relationship between these quantities when using dimer constructs. The G ratio corresponds to the slope of a linear regression line, while the F ratio is calculated from the y-axis offset. (C) Left: cartoon of the dimer constructs. Right: Plot of FRET efficiencies determined from donor quenching ( E D ) vs. A free for the shown dimers. FRET calibration results in stable FRET efficiencies independent of the fluorophore concentration ( A free or D free ). The plot for sensitized emission ( E A ) should look similar. All calibration images were processed with the “FRET_PIX” macro instead of the “FRET_ROI” macro.
    Spectral Correction Mtq2 (Donor Only), supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/spectral+correction+mtq2+(donor+only)/pmturquoise2+golgi/pmc10400964-0-8-21
    Average 90 stars, based on 1 article reviews
    spectral correction mtq2 (donor only) - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Expected outcomes: Spectral correction factors and FRET calibration (A) Determination of spectral correction factors: R A1 : Plot of fluorescence in the FRET channel (y-axis) vs. fluorescence in the YFP channel (x-axis). R A1 describes cross excitation of YFP by CFP excitation (FRET/YFP). R D1 : Plot of fluorescence in the FRET channel (y-axis) vs. fluorescence in the CFP channel (x-axis). R D1 describes the bleed-through of CFP emission into the FRET emission detection range (FRET/CFP). R D2 : Plot of fluorescence in the YFP channel vs. the fluorescence in the CFP channel. R D2 describes cross excitation of CFP from the YFP excitation (YFP/CFP). These spectral correction factors are determined by linear regressions between the specified channels. (B) Correlating sensitized emission versus donor quenching displays an affine relationship between these quantities when using dimer constructs. The G ratio corresponds to the slope of a linear regression line, while the F ratio is calculated from the y-axis offset. (C) Left: cartoon of the dimer constructs. Right: Plot of FRET efficiencies determined from donor quenching ( E D ) vs. A free for the shown dimers. FRET calibration results in stable FRET efficiencies independent of the fluorophore concentration ( A free or D free ). The plot for sensitized emission ( E A ) should look similar. All calibration images were processed with the “FRET_PIX” macro instead of the “FRET_ROI” macro.

    Journal: STAR Protocols

    Article Title: Protocol for deriving proximity, affinity, and stoichiometry of protein interactions using image-based quantitative two-hybrid FRET

    doi: 10.1016/j.xpro.2023.102459

    Figure Lengend Snippet: Expected outcomes: Spectral correction factors and FRET calibration (A) Determination of spectral correction factors: R A1 : Plot of fluorescence in the FRET channel (y-axis) vs. fluorescence in the YFP channel (x-axis). R A1 describes cross excitation of YFP by CFP excitation (FRET/YFP). R D1 : Plot of fluorescence in the FRET channel (y-axis) vs. fluorescence in the CFP channel (x-axis). R D1 describes the bleed-through of CFP emission into the FRET emission detection range (FRET/CFP). R D2 : Plot of fluorescence in the YFP channel vs. the fluorescence in the CFP channel. R D2 describes cross excitation of CFP from the YFP excitation (YFP/CFP). These spectral correction factors are determined by linear regressions between the specified channels. (B) Correlating sensitized emission versus donor quenching displays an affine relationship between these quantities when using dimer constructs. The G ratio corresponds to the slope of a linear regression line, while the F ratio is calculated from the y-axis offset. (C) Left: cartoon of the dimer constructs. Right: Plot of FRET efficiencies determined from donor quenching ( E D ) vs. A free for the shown dimers. FRET calibration results in stable FRET efficiencies independent of the fluorophore concentration ( A free or D free ). The plot for sensitized emission ( E A ) should look similar. All calibration images were processed with the “FRET_PIX” macro instead of the “FRET_ROI” macro.

    Article Snippet: Spectral correction R A1 , R D1 , R D2 , mTq2 (donor only) mVen (acceptor only) , mTq2 mVen , AddGene#198196 AddGene#198192.

    Techniques: Fluorescence, Construct, Concentration Assay